Career Center

Description

SUBFUNCTION DEFINITION: The Malik lab at Cincinnati Children's Hospital Medical Center has led many gene therapy studies on hematopoietic stem cell disorders, such as hemoglobinopathies (thalassemia and sickle cell disease), immune deficiency (X-SCID) and immune dysregulatory disorders (HLH) and using a variety of viral vectors and have translated these efforts with retroviral and lentiviral vectors into clinical trials for these diseases. The lab is now focused on gene editing for hematopoietic diseases, with a focus on designing platforms that primarily mediate homology directed repair.

REPRESENTATIVE RESPONSIBILITIES

???Perform experiments and record data
Follow established research protocols. Understand the scientific principles involved in the research. Apply those principles to troubleshoot and modify procedures and techniques when appropriate. Maintain accurate and detailed records of all laboratory work and experiments. Analyze data and perform statistical and graphical analysis of data. Design and execute daily laboratory experiments in coordination with senior laboratory personnel. Apply standard protocols to specific experiments. Assists in training post doctoral fellows, graduate students, and junior laboratory personnel in laboratory techniques and equipment. Capable of directing an undergraduate trainee in simple, well-established procedures. Maintains an active role in laboratory's meetings, presentations, and publications. Contributes material for reports, publications, journal articles, and abstracts. Able to orally present data in group presentations.

???Research and improve current methods
Utilize internal and external resources to reproduce, evaluate, research, and improve techniques, bring in new techniques or modify existing techniques. Evaluate innovative techniques

???Lab Administration
Troubleshoot and correct erroneous results or problems with laboratory equipment. Troubleshoot and/or make modifications as necessary to established procedures. Maintain inventory of laboratory supplies and equipment. Maintain cell cultures. Operate standard laboratory equipment. Willing and able to assist in daily operations of the laboratory including answering phone, maintaining lab supplies, organization, and cleanliness. Maintains optimal protocol and assay accuracy by conducting regular quality checks. Provides appropriate safety monitoring checks as assigned (radiation safety, biosafety, etc.)
???Serve as a Resource
Demonstrate competence in specific knowledge, skills and behaviors. Effectively communicate with team members and contribute to their growth by sharing knowledge, experience, and skills. Stay abreast of changes in key systems. Serve as a resource to the department in specific area(s).

???Customer Service
Maintain/manage strong working relationships with clients, colleagues, and other key stakeholders. Respond to requests quickly and effectively. Identify and understand customer needs and expectations and meets them. Communicate any relevant information to clients, colleagues, and other key stakeholders.

Qualifications

EDUCATION/EXPERIENCE

Required for RAII:

??? High school diploma or equivalent

??? 2 years of work experience in a related job discipline. or equivalent combination of education and experience

???May require an Associate degree, vocational training, apprenticeships or equivalent

Required for RAIII:

??? Bachelor's degree in a related field

??? 1 year of work experience in a related job discipline OR Master's degree in a related field

Required for RAIV:

??? Bachelor's degree in a related field

??? 3 years of experience in related job discipline OR Master's degree and 2 years experience

Preferred:

We are a lab performing gene therapy and gene editing for hematopoietic disorders and work with vectors, CRISPR/Cas9 and use human and mouse models of hematopoiesis and diseases to discover and translate therapies to human diseases. Our lab also supports clinical trials and performs assays on clinical trial samples.

We are seeking an RAII/III/IV technician who has experience in coordinating and sample processing of clinical trial samples, performing ELISAs, DNA PCRs. Has experience in mouse breeding and colony maintenance, performing rodent handling and procedures, mouse genotyping (DNA extraction from clipped mouse tails and blood samples, genotyping PCRs, gel electrophoresis), maintaining and updating electronic Mouse Colony Database and Human Trial electronic databases. We require the potential candidate to be savvy in computer software (word, excel, powerpoint, access).Experience in Flow cytometry, molecular cloning and cell culture will be desirable. Ability to work meticulously, as part of a team with good communication and organization skills is highly desirable.